Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of white blood cells in the peripheral blood (x10 9 /l) and the ratio of CD34 + cells among the mononuclear cells (%).

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Effects of autologous bone marrow-derived stem cell mobilization on acute tubular necrosis and cell apoptosis in rats

doi: 10.3892/etm.2015.2592

Figure Lengend Snippet: Number of cluster of differentiation 34+ cells in four groups of rat renal tissues (arrows indicate immunohistochemical staining; magnification, x200). (A–C) The (A) normal control, (B) treatment control and (C) model groups at day 5; (D–F) the model group at (D) day 10, (E) day 17 and (F) day 24; (G–J) the treatment group at (G) day 5, (H) day 10, (I) day 17 and (J) day 24.

Article Snippet: Rabbit anti-rat CD34 + polyclonal antibody (dilution, 1:200; cat. no. bs-0754R; Beijing Bioss Biosynthesis Biotechnology Co., Ltd.) was used as the primary antibody for overnight incubation at 4°C.

Techniques: Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Morphofunctional State and Circadian Rhythms of the Liver of Female Rats under the Influence of Chronic Alcohol Intoxication and Constant Lighting

doi: 10.3390/ijms231810744

Figure Lengend Snippet: Levels of p53 expression in hepatocytes of rats. *** ( p ≤ 0.0005)—in comparison with the parameters of animals of the control group.

Article Snippet: The following antibodies were used: Ki-67–Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300; PER2 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; BMAL1 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; p53 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; ADH5 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Morphofunctional State and Circadian Rhythms of the Liver of Female Rats under the Influence of Chronic Alcohol Intoxication and Constant Lighting

doi: 10.3390/ijms231810744

Figure Lengend Snippet: Expression of p53 in liver of rats. ( A )—Control; ( B )—EtOH; ( C )—CL; ( D )—EtOH + CL. ×200.

Article Snippet: The following antibodies were used: Ki-67–Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300; PER2 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; BMAL1 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; p53 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; ADH5 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Morphofunctional State and Circadian Rhythms of the Liver of Female Rats under the Influence of Chronic Alcohol Intoxication and Constant Lighting

doi: 10.3390/ijms231810744

Figure Lengend Snippet: Results of the cosinor analysis of the daily dynamics of the expression of the studied genes.

Article Snippet: The following antibodies were used: Ki-67–Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300; PER2 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; BMAL1 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; p53 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; ADH5 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Morphofunctional State and Circadian Rhythms of the Liver of Female Rats under the Influence of Chronic Alcohol Intoxication and Constant Lighting

doi: 10.3390/ijms231810744

Figure Lengend Snippet: Daily dynamics of p53 expression in hepatocytes of rats.

Article Snippet: The following antibodies were used: Ki-67–Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300; PER2 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; BMAL1 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; p53 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:200; ADH5 –Rabbit polyclonal (Cloud-Clone Corp., Houston, TX, USA), 1:300.

Techniques: Expressing

Journal: Journal of the Endocrine Society

Article Title: The Orphan Nuclear Receptor Liver Homolog Receptor-1 (Nr5a2) Regulates Ovarian Granulosa Cell Proliferation

doi: 10.1210/js.2017-00329

Figure Lengend Snippet: Proliferation of mouse granulosa cells is compromised in Nr5a2-depleted follicles. (A, left) Amhr2 Cre depletion of Nr5a2 transcripts in granulosa cells from mouse antral follicles (n = 5). (A, middle and right) BrdU and PCNA expression in CON and cKO granulosa cells at 44 h after gonadotropin treatment, as evaluated by quantitative image analysis. Data are means ± standard error (SE); * P < 0.05, *** P < 0.001. (B) Representative images showing the expression of BrdU in CON and cKO ovaries at 44 hours after gonadotropin stimulation in vivo (n = 10/n = 6). (C) Representative images for PCNA at 44 hours after gonadotropin stimulation (n = 4). In this and subsequent figures, CON signifies control animals, cKO the granulosa-specific mouse knockout line (genotype Nr5a2f/fAmhr2 Cre/+ ). Original scale bars, 50 µM.

Article Snippet: We then treated with a rabbit polyclonal antibody against PCNA (SA194; Biomol, Hamburg, Germany): first antibody 1:200 in BSA 5%/PBS at 4°C overnight and with cyanine-3 (Cy3)-conjugated goat anti-rabbit IgG second antibody (111-165-144; Jackson ImmunoResearch Laboratories), diluted 1:400 in PBS 1× for 1 hour at room temperature.

Techniques: Expressing, In Vivo, Control, Knock-Out

Journal: Journal of the Endocrine Society

Article Title: The Orphan Nuclear Receptor Liver Homolog Receptor-1 (Nr5a2) Regulates Ovarian Granulosa Cell Proliferation

doi: 10.1210/js.2017-00329

Figure Lengend Snippet: FSHr is elevated in cKO granulosa cells, and exogenous estrogen cannot rescue proliferation relative it CON animals. (A, left) Abundance of FSHr transcripts in CON and cKO mice following gonadotropin stimulation (n = 5). (A, middle) The number of proliferating cells following 1 mg estrogen administration in vivo , as determined by quantitative imaging of BrdU expression. (A, right) The quantitative analysis of PCNA expression between granulosa cells from CON and cKO animals. Data are expressed as means ± SE of n = 4 animals/group. * P < 0.05. (B) Representative photomicrographs of BrdU incorporation in ovaries from estrogen-treated CON and cKO mice. (C) Representative photomicrographs showing PCNA expression in the ovaries of estrogen-treated CON and cKO mice. Original scale bars, 50 µM.

Article Snippet: We then treated with a rabbit polyclonal antibody against PCNA (SA194; Biomol, Hamburg, Germany): first antibody 1:200 in BSA 5%/PBS at 4°C overnight and with cyanine-3 (Cy3)-conjugated goat anti-rabbit IgG second antibody (111-165-144; Jackson ImmunoResearch Laboratories), diluted 1:400 in PBS 1× for 1 hour at room temperature.

Techniques: In Vivo, Imaging, Expressing, BrdU Incorporation Assay

Journal: Open Medicine

Article Title: Echinacoside inhibits PASMCs calcium overload to prevent hypoxic pulmonary artery remodeling by regulating TRPC1/4/6 and calmodulin

doi: 10.1515/med-2024-1044

Figure Lengend Snippet: Effect of ECH on pulmonary artery remodeling in HPH rats. (a)–(d) HE staining results of lung tissue of rats in each group, (e) WT%, (f) pulmonary vascular lumen area/total wall area (WA%), (g) pulmonary vascular lumen area/total wall area (LA%), and (h)–(k) results of immunofluorescence staining of lung tissue in each group, DAPI stained the nucleus blue; PCNA showed positive expression green; mainly expressed in the nucleus. The positive expression of α-SMA is red and is mainly expressed in the cytoplasm. (l) Number of positive co-expressing cells stained by immunofluorescence. 1) p < 0.05 vs normoxic group; 2) p < 0.05 vs HPH group; 3) p < 0.05 vs HPH + ECH (15 mg/kg) group ( n = 6).

Article Snippet: Proliferating cell nuclear antigen (PCNA) polyclonal antibody (10205-2-AP) was supplied from Wuhan Sanying Biotechnology Co., Ltd (Wuhan, China).

Techniques: Staining, Immunofluorescence, Expressing